Interaction of Orexin and Bone Morphogenetic Proteins in Steroidogenesis by Human Adrenocortical Cells

Orexins are neuropeptides that play important roles in sleep-wake regulation and food intake the central nervous system, but their receptors also expressed peripheral tissues, including endocrine system. In present study, we investigated functions of orexin adrenal steroidogenesis using human adrenocortical H295R cells by focusing on its interaction with bone morphogenetic proteins (BMPs) induce steroidogenesis. Treatment A increased mRNA levels steroidogenic enzymes StAR, CYP11B2, CYP17, HSD3B1, these effects were further enhanced presence forskolin. Interestingly, treatment suppressed BMP-receptor signaling detected Smad1/5/9 phosphorylation Id-1 expression through upregulation inhibitory Smad7. Orexin endogenous BMP-6 type-II receptor ActRII cells. Moreover, downregulated level OX1R, not OX2R, conclusion, results indicate both accelerate cells; pathways mutually inhibit each other, thereby leading to a fine-tuning